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Sunday, July 19, 2020 | History

2 edition of fine-structural analysis of in vitro myogenesis of mutant (crooked neck dwarf) chick found in the catalog.

fine-structural analysis of in vitro myogenesis of mutant (crooked neck dwarf) chick

Francis John Traeger

fine-structural analysis of in vitro myogenesis of mutant (crooked neck dwarf) chick

by Francis John Traeger

  • 206 Want to read
  • 21 Currently reading

Published .
Written in English

    Subjects:
  • Myogenesis,
  • Muscle,
  • Chickens -- Embryos

  • Edition Notes

    Statementby Francis John Traeger
    The Physical Object
    Pagination123 leaves :
    Number of Pages123
    ID Numbers
    Open LibraryOL14445884M

    The analysis of myogenin knockout mice revealed that the expression of several differentiation markers, such as myosin heavy chain and MRF4, was reduced, whereas MyoD levels were normal (Hasty et al. ). Phenotypically, this resulted in normal somitic compartmentalization during development but manifested in diffuse myofiber formation and an abundance of undifferentiated myoblasts in the later . Cell Cycle Regulation in Myogenesis. Consistent with these in vitro findings, muscle regeneration is delayed in Fkbp5−/− mice. This analysis has allowed us to identify those residues.

    Molecular analysis of a Drosophila minichromosome, Dp(1;f), revealed a relationship between position-effect variegation and the copy number reductions of heterochromatic sequences that occur in polytene cells. Heterochromatin adjacent to a defined junction . Recapitulating these in vitro observations, the process of muscle regeneration following injury in young adult mice was accelerated by Cxcl14 depletion, accompanied by reduced cell proliferation.

      Semaphorin III is a membrane-associated secreted protein with a developmentally dynamic pattern of expression, including particular domains of the nervous system, the borders of developing bones Cited by:   The generation and characterization of mutants is an essential component of any study on structure-- function relationships. Knowledge of the threedimensional structure of .


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Fine-structural analysis of in vitro myogenesis of mutant (crooked neck dwarf) chick by Francis John Traeger Download PDF EPUB FB2

a fine-structural analysis of the fusion of myogenic cells Bruce H. Lipton and Irwin R. Konigsberg From the Department of Biology, The University of Virginia Cited by: After DNA synthesis has been mediated by DNA polymerase in vitro, the M13 DNA is allowed to replicate in E.

coli, in which case many of the resulting phages will be the desired mutant. The synthetic oligonucleotide can be used as a labeled probe to distinguish wild-type from mutant : Anthony Jf Griffiths, Jeffrey H Miller, David T Suzuki, Richard C Lewontin, William M Gelbart.

DEVELOPMENTAL BIOL () A Fine-Structural Analysis of Normal and Modulated Cells in Myogenic Cultures BRUCE H. LIPTON Department of Anatomy, University of Wisconsin, Madison, Wisconsin Received Febru ; accepted in revised form It has formerly been reported that muscle and nonmuscle progenitor cells in Cited by: DEVELOPMENTAL BIOL () An in Vitro Study of Normal and Mutant Myogenesis in the Mouse1'2 FRANCINE BOWDEN-ESSIEN Department of Genetics, Albert Einstein College of Medicine Bronx, New York3 Accepted Octo Precursor muscle cells from embryonic mice homozygous for the mutation muscular dysgen- esis (mdglmdg) differentiate into cross-striated myotubes in monodispersed cell Cited by: A fine structural analysis of fusion in myogenic cells Article (PDF Available) in The Journal of Cell Biology 53(2) June with Reads How we measure 'reads'.

Dev Biol. Mar;27(3) An in vitro study of normal and mutant myogenesis in the mouse. Bowden-Essien F. PMID: [Indexed for MEDLINE]Cited by: Kinetic analysis of myogenesis in vitro Article (PDF Available) in The Journal of Cell Biology 52(1) February with 10 Reads How we measure 'reads'.

In vitro mutagenesis, plant regeneration and characterization of mutants via RAPD analysis in Baby's breath Gypsophila paniculata L.

Barakat*1 and Heba El-Sammak2 1Plant Genetic Manipulation and Genomic Breeding Group, Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia. This later group of cardiac tissues served as control materials for fine structural studies.

The ventricular pieces were first fixed for 1^-3 hr at room temperature with modified K-arnovsky's fixative (), which consisted of a mixture of 4% paraformaldehyde and 4% glutaralde- hyde in M cacodylate buffer adjusted to pH Cited by: Development of an in vitro myogenesis assay Anna Arnaud University of Arkansas, Fayetteville Analysis of PCR results showed negligible amounts of MyoD present in all experimental wells.

of MyoD to create an in vitro myogenesis assay for use in. Francis John Traeger has written: 'A fine-structural analysis of in vitro myogenesis of mutant (crooked neck dwarf) chick' -- subject(s): Muscle, Chickens, Embryos, Myogenesis Asked in Actors. Normal growth and repair of skeletal muscle is mediated by a precursor population termed satellite cells and myogenesis by these cells both in vivo and in vitro has been well documented.

– However, in regenerating muscle, the number of myogenic precursors exceeds that of resident satellite cells implying recruitment and migration of progenitors from other sources, such as the bone marrow.

Citation: Hwang Y, Suk S, Lin S, Tierney M, Du B, et al. () Directed In Vitro Myogenesis of Human Embryonic Stem Cells and Their In Vivo Engraftment. PLoS ONE 8(8): e doi/journal. Extensive analysis of Myf5 nlacZ/nlacZ:Myod-/-mutants failed to demonstrate skeletal myogenesis with a number of muscle markers at all developmental stages 4 (data not shown).Cited by:   Myogenic cell lines have proved to be useful tools for investigating the molecular mechanisms that control cellular differentiation.

NFB-s is a mutant myogenic cell line which fails to differentiate in vitro, and can repress differentiation in normal myogenic cells when fused to form heterokaryons. The NFB-s cell line was used here to study the molecular mechanisms underlying Author: Daniel K.

Rohrer, Helen M. Blau. We established the in vivo relevance of adenylyl cyclase (AC) pathway activity in myogenesis by assaying for a downstream effector of this cascade, CREB, in embryonic regions undergoing myogenesis. CREB is the founding member of the CREB family of basic leucine zipper transcription factors (including ATF-1 and CREM).

MyoD is a master regulator of myogenesis with a potent ability to redirect the cell fate of even terminally differentiated cells. Hence, enhancing the activity of MyoD is an important step to maximising its potential utility for in vitro disease modelling and cell replacement therapies.

We have previously shown that the reprogramming activity of several neurogenic bHLH proteins can be Cited by: 9. The Rb promoter region responsible for the regulation during in vitro myogenesis (Okuyama et al.

) was analyzed. Proliferating and 48 h-differentiated cells showed different patterns of nucleotide protection (Fig. 5 c), supporting the idea of the presence of different transcriptional complexes in the two conditions by: fusion of the genetic defect of the mdg mouse mutant muscle fibers (Chaudary et al., ; Courbin et al., ).

Subsequently, several groups reported that genet-ically labeled dermal fibroblasts could be incorporated into differentiated myotubes both in vitro and in. About 20 years ago, molecular genetic analysis of tissue culture responses with temperature-sensitive mutants of Arabidopsis was launched in the laboratory of Prof.

Atsushi Komamine as a new approach to mechanisms of plant organogenesis in vitro. In this and later studies, many interesting mutants were isolated and characterized, which led to the identification of unexpected Cited by: 6.

We identify Fgf8 as a major relay factor in RA-mediated activation of myogenesis. We show that fgf8 expression in somites and anterior psm is regulated by RA, and find that in the absence of Fgf8 signalling in the acerebellar mutant RA fails to promote myoD expression.

Bader, D., Masaki, T., and Fischman, D. A. () Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro, J Cell B – PubMed CrossRef Google Scholar Cited by: 7.

MUTANTS of the dystrophia muscularis gene (dy) in mouse suffer from progressive skeletal muscle loss, together with inadequate repair or regeneration6. In I .